EVALUASI POTENSI ANTIOKSIDAN EKSTRAK DAUN MANGGA ARUMANIS (Mangifera indica L. var. Arumanis) DALAM SISTEM NANOPARTIKEL KITOSAN DENGAN UJI DPPH

Abstract

Oxidative stress is a key contributor to the pathogenesis of degenerative diseases, highlighting the need for effective natural antioxidants with enhanced bioavailability. This study aimed to develop a chitosan-based nanoparticle system containing Mangifera indica var. arumanis leaf extract and to evaluate its antioxidant activity. Nanoparticle synthesis was achieved via ionic gelation, followed by morphological characterization using Scanning Electron Microscopy (SEM). Antioxidant efficacy was evaluated through the DPPH assay by calculating IC?? values, with ascorbic acid serving as the reference standard. The ethanolic extract yielded an IC?? of 94.92 ppm. In contrast, the chitosan nanoparticle formulation significantly improved antioxidant performance, achieving an IC?? of 80.05 ppm. Ascorbic acid showed the highest antioxidant activity with an IC?? value of 4.86 ppm. These findings indicate that the chitosan nanoparticle system improves the antioxidant effectiveness of Mangifera indica var. arumanis leaf extract and has potential for development as a natural antioxidant agent.